Phytotherapy research : PTR · 2006
Effects of ephedrae herba on melanogenesis and gene expression profiles using cDNA microarray in B16 melanocytes.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Kim Hyeong Jun, Cho Yeong Do, Leem Kang-Hyun, Lee Dong-Nyung, Kim Ee-Hwa, Kim Myung-Gyou, Kim Dae-Keun, Shin Tae-Yong, Boo Yungmin, Lee Je-Hyun, Kim Hye Kyung
Аннотация
Ephedrae Herba (EH) is obtained by drying the stems of Ephedra sinica Stapf (family Ephedraceae). EH has been used clinically to treat colds and to reduce edema in skin. The effects of EH on melanogenesis were studied in B16 murine melanocytes. The tyrosinase activity and melanin content were measured after incubation with EH. Both reverse transcription-polymerase chain reaction and cDNA microarray analysis were used to study the mechanism of EH action. EH decreased tyrosinase activity and melanin content in a dose-dependent manner. EH decreased microphthalmia-associated transcription factor (MITF) and tyrosinase gene expression. These effects were controlled by MITF-mediated regulation of tyrosinase gene expression. EH also altered the expression of about 100 other genes. These results suggest that EH may be used clinically to treat freckles and liver spots.