Virulence of Babesia bigemina in infected cattle (Bos taurus): Molecular and immunological studies.

This study examined 400 tick-infested cattle from the following four governorates in Egypt: Faiyum, Beni Suef, Giza, and Minya. These cattle were examined for blood parasites between January 2021 and April 2022. The infected cattle were classified into four groups based on tick infestations and clinical signs. Blood was drawn for assessing oxidative stress markers as well as for parasitological examination and molecular analysis of the 18S rRNA gene of Babesia bigemina (B. bigemina). We performed a comparison of the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) between B. bigemina-infected blood samples and non-infected blood samples used as negative controls. Babesia spp. infection increases hemolysis, which in turn increases oxidative stress marker levels and cell-mediated immune response.