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DOI 10.1016/j.clim.2024.109942

Медицинская библиотека · Clinical immunology (Orlando, Fla.) · 2024

Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia.

Abraham Roshini S, Basu Amrita, Heimall Jennifer R, Dunn Elizabeth, Yip Alison, Kapadia Malika, Kapoor Neena, Satter Lisa Forbes, Buckley Rebecca, O'Reilly Richard, Cuvelier Geoffrey D E, Chandra Sharat, Bednarski Jeffrey, Chaudhury Sonali, Moore Theodore B, Haines Hilary, Dávila Saldaña Blachy J, Chellapandian Deepakbabu, Rayes Ahmad, Chen Karin, Caywood Emi, Chandrakasan Shanmuganathan, Lugt Mark Thomas Vander, Ebens Christen, Teira Pierre, Shereck Evan, Miller Holly, Aquino Victor, Eissa Hesham, Yu Lolie C, Gillio Alfred, Madden Lisa, Knutsen Alan, Shah Ami J, DeSantes Kenneth, Barnum Jessie, Broglie Larisa, Joshi Avni Y, Kleiner Gary, Dara Jasmeen, Prockop Susan, Martinez Caridad, Mousallem Talal, Oved Joseph, Burroughs Lauri, Marsh Rebecca, Torgerson Troy R, Leiding Jennifer W, Pai Sung Yun, Kohn Donald B, Pulsipher Michael A, Griffith Linda M, Notarangelo Luigi D, Cowan Morton J, Puck Jennifer, Dvorak Christopher C, Haddad Elie

СтатьяПоддержка NIH: внешняя программаПеревод пока не подготовлен

Аннотация

Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had <10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/μL, there was a higher proliferative response with the PHA flow assay compared to the 3H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID.

Для цитирования: Abraham Roshini S, Basu Amrita, Heimall Jennifer R, Dunn Elizabeth, Yip Alison, Kapadia Malika, Kapoor Neena, Satter Lisa Forbes, Buckley Rebecca, O'Reilly Richard, Cuvelier Geoffrey D E, Chandra Sharat, Bednarski Jeffrey, Chaudhury Sonali, Moore Theodore B, Haines Hilary, Dávila Saldaña Blachy J, Chellapandian Deepakbabu, Rayes Ahmad, Chen Karin, Caywood Emi, Chandrakasan Shanmuganathan, Lugt Mark Thomas Vander, Ebens Christen, Teira Pierre, Shereck Evan, Miller Holly, Aquino Victor, Eissa Hesham, Yu Lolie C, Gillio Alfred, Madden Lisa, Knutsen Alan, Shah Ami J, DeSantes Kenneth, Barnum Jessie, Broglie Larisa, Joshi Avni Y, Kleiner Gary, Dara Jasmeen, Prockop Susan, Martinez Caridad, Mousallem Talal, Oved Joseph, Burroughs Lauri, Marsh Rebecca, Torgerson Troy R, Leiding Jennifer W, Pai Sung Yun, Kohn Donald B, Pulsipher Michael A, Griffith Linda M, Notarangelo Luigi D, Cowan Morton J, Puck Jennifer, Dvorak Christopher C, Haddad Elie. Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia.. Clinical immunology (Orlando, Fla.). 2024. DOI: 10.1016/j.clim.2024.109942.

Предметные рубрики MeSH

Infant, NewbornHumansSevere Combined ImmunodeficiencyLymphopeniaNeonatal ScreeningT-LymphocytesCell Proliferation
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