Genotyping of human cystic echinococcosis in northeastern Iran.

Cystic echinococcosis (CE) is a human public health problem in northeast of Iran and there is limited information regarding the genotypes of Echinococcus granulosus senso lato (s.l.) in human in this region. In the present study, we determined the genotypes of E. granulosus s.l. infecting human in northeastern Iran from Khorasan Razavi province using PCR-RFLP of rDNA ITS1 region. From April 2013 to December 2016, 50 hepatic hydatid cysts were recovered from 50 patients who underwent surgical procedure. Protoscoleces were collected from individual cysts, DNA was extracted, and the rDNA ITS1 gene was amplified by PCR and genotypes confirmed by RFLP. All the cyst materials were identified as Echinococcus granulosus senso stricto (s.s.), indicating this species as the main cause of CE in humans in northeast of Iran.