[Generation of human Fab antibody against platelet membrane glycoprotein IIb/IIIa and its effect on platelet aggregation].

AIM: To generation of human Fab fragment against GP GPIIb/IIIa from phage display library and to study its effect on platelet aggregation.

METHODS

An antibody phage display library was constructed from spleen cells from a donor with idiopathic thrombocytopenic purpura(ITP) whose anti-GPIIb/IIIa antibody was positive. High-affinity human mAbs was selected by panning against GPIIb/IIIa expressed on CHO123 cells. The binding specificity of phage antibody to GPIIb/IIIa was detected by ELISA and Western blot. And the effect of antibody on platelet aggregation was analyzed.

RESULTS

After three rounds of panning with CHO123, phage antibodies against GPIIb/IIIa were enriched specifically. 2 positive phage antibodies with high specific for GP IIb/IIIa were verified, and the purified antibody can inhibited ADP induced platelet aggregation.

CONCLUSION

Human antibodies against GPIIb/IIIa are obtained from phage display library.