World journal of virology · 2026
Study of extended-spectrum beta-lactamases and AmpC beta-lactamases producing Klebsiella from clinical samples in tertiary care hospital of Punjab.
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Kaur Harpreet, Arora Shilpa, Sharma Vishal, Kaur Kirandeep, Kamboj Shivani
Аннотация
BACKGROUND: Klebsiella species are important opportunistic pathogens responsible for various hospital- and community-acquired infections. The rise of multidrug-resistant strains, especially those producing extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases, poses a major challenge to effective antimicrobial therapy and infection control.
AIM: To determine the antimicrobial susceptibility pattern of Klebsiella isolates and to detect the presence of ESBL and AmpC beta-lactamases.
METHODS: A one-year cross-sectional study was conducted on 920 Gram-negative isolates, from which 130 non-repetitive Klebsiella isolates (14.13%) were selected. Antimicrobial susceptibility testing was performed, and ESBL and AmpC production were identified using standard phenotypic confirmatory methods.
RESULTS: Among 130 Klebsiella isolates, Klebsiella pneumoniae was the predominant species (n = 92, 70.76%). Most isolates were obtained from patients aged 45-60 years (n = 33, 25.38%) and from pus samples (n = 55, 42.3%), with the highest frequency from surgical departments (n = 36, 27.7%). Among the tested antibiotics, gentamicin showed the greatest susceptibility (n = 81, 62.3%). ESBL production was detected in 77 isolates (59.2%), while 62 isolates (47.69%) produced AmpC beta-lactamases. Co-production of ESBL and AmpC was found in 35 isolates (26.92%).
CONCLUSION: Klebsiella species are significant nosocomial and opportunistic pathogens characterized by high multidrug resistance, often through the production of extended-spectrum and AmpC beta-lactamases. Surveillance, confirmatory testing, and infection control guide antibiotic use and prevent spread.
AIM: To determine the antimicrobial susceptibility pattern of Klebsiella isolates and to detect the presence of ESBL and AmpC beta-lactamases.
METHODS: A one-year cross-sectional study was conducted on 920 Gram-negative isolates, from which 130 non-repetitive Klebsiella isolates (14.13%) were selected. Antimicrobial susceptibility testing was performed, and ESBL and AmpC production were identified using standard phenotypic confirmatory methods.
RESULTS: Among 130 Klebsiella isolates, Klebsiella pneumoniae was the predominant species (n = 92, 70.76%). Most isolates were obtained from patients aged 45-60 years (n = 33, 25.38%) and from pus samples (n = 55, 42.3%), with the highest frequency from surgical departments (n = 36, 27.7%). Among the tested antibiotics, gentamicin showed the greatest susceptibility (n = 81, 62.3%). ESBL production was detected in 77 isolates (59.2%), while 62 isolates (47.69%) produced AmpC beta-lactamases. Co-production of ESBL and AmpC was found in 35 isolates (26.92%).
CONCLUSION: Klebsiella species are significant nosocomial and opportunistic pathogens characterized by high multidrug resistance, often through the production of extended-spectrum and AmpC beta-lactamases. Surveillance, confirmatory testing, and infection control guide antibiotic use and prevent spread.