Frontiers in bioengineering and biotechnology · 2026
Metformin-loaded J-AuPPS for infected diabetic wound treatment.
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Yin Fuli, Yang Lingkai, Shan Haojie, Li Yuange, Zhou Yongfeng, Yu Xiaowei
Аннотация
INTRODUCTION: Infected diabetic wounds pose a significant clinical challenge owing to the complex wound microenvironment. Various multifunctional synergistic therapeutic nanomaterials have been successfully developed to treat diabetic infected wounds.
OBJECTIVE: A Janus Au-porphyrin polymersome heterostructure loaded with metformin (Met@J-AuPPS) is constructed and presented.
METHODS: Porphyrin polymersome vesicles loaded with metformin (Met@PPS) was synthesized by supramolecular polymerization enhanced self-assembly. Metformin-loaded Janus gold-porphyrin polymersome (Met@J-AuPPS) was synthesized by photocatalytic synthesis method. Transmission electron microscope (TEM), scanning electron microscope (SEM), UV-vis absorption spectrum and ELISA experiment were used to detect the morphology, structure, photothermal properties and drug release properties of Met@J-AuPPS. The effects of Met@J-AuPPS on the proliferation and activity of HUVECs were detected by CCK-8 test, scratch test, Transwell test and tube formation test. The antibacterial and antibiofilm abilities of Met@J-AuPPS were detected by blood plate test, crystal violet staining, SEM of biofilm and bacterial live/dead staining. Imaging and histological staining were used to detect the efficacy of Met@J-AuPPS in treating diabetic wound infections in vivo.
RESULTS: Excellent photothermal antibacterial performance against gram-positive methicillin-resistant Staphylococcus aureus (MRSA), gram-negative extended-spectrum β-lactamases Escherichia coli (ESBL E. coli), and MRSA/ESBL E. coli biofilm is demonstrated by a strong non-centrosymmetric near-field enhancement (NFE) effect between Met@PPS and Au nanoparticles. Furthermore, the release of metformin under near-infrared (NIR) light promotes angiogenesis and tissue repair. The results therefore show that Met@J-AuPPS exhibits excellent antibacterial/biofilm and pro-angiogenic performance, with significantly enhanced therapeutic effects in infected wounds of diabetic rat models.
CONCLUSION: This study presents an innovative therapeutic strategy for diabetic wound infections and demonstrates that Met@J-AuPPS has potential as a multifunctional and upgradeable nanoplatform for further biomedical applications.
OBJECTIVE: A Janus Au-porphyrin polymersome heterostructure loaded with metformin (Met@J-AuPPS) is constructed and presented.
METHODS: Porphyrin polymersome vesicles loaded with metformin (Met@PPS) was synthesized by supramolecular polymerization enhanced self-assembly. Metformin-loaded Janus gold-porphyrin polymersome (Met@J-AuPPS) was synthesized by photocatalytic synthesis method. Transmission electron microscope (TEM), scanning electron microscope (SEM), UV-vis absorption spectrum and ELISA experiment were used to detect the morphology, structure, photothermal properties and drug release properties of Met@J-AuPPS. The effects of Met@J-AuPPS on the proliferation and activity of HUVECs were detected by CCK-8 test, scratch test, Transwell test and tube formation test. The antibacterial and antibiofilm abilities of Met@J-AuPPS were detected by blood plate test, crystal violet staining, SEM of biofilm and bacterial live/dead staining. Imaging and histological staining were used to detect the efficacy of Met@J-AuPPS in treating diabetic wound infections in vivo.
RESULTS: Excellent photothermal antibacterial performance against gram-positive methicillin-resistant Staphylococcus aureus (MRSA), gram-negative extended-spectrum β-lactamases Escherichia coli (ESBL E. coli), and MRSA/ESBL E. coli biofilm is demonstrated by a strong non-centrosymmetric near-field enhancement (NFE) effect between Met@PPS and Au nanoparticles. Furthermore, the release of metformin under near-infrared (NIR) light promotes angiogenesis and tissue repair. The results therefore show that Met@J-AuPPS exhibits excellent antibacterial/biofilm and pro-angiogenic performance, with significantly enhanced therapeutic effects in infected wounds of diabetic rat models.
CONCLUSION: This study presents an innovative therapeutic strategy for diabetic wound infections and demonstrates that Met@J-AuPPS has potential as a multifunctional and upgradeable nanoplatform for further biomedical applications.