Frontiers in veterinary science · 2026
Genetic diversity with potential ESBL-producing and multidrug-resistant Salmonella strains from chicken meat.
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Orhan Selman Bahadır, Suleymanoglu Ali Anil, Aydin Ali
Аннотация
OBJECTIVES: Salmonella, a major foodborne pathogen, is a primary concern due to its role in spreading antibiotic resistance. Raw chicken meat samples (n = 210) were collected from various retail locations in Istanbul.
METHODS: The food samples were isolated according to ISO 6579-1 and 13 (6.2%) of Salmonella strains confirmed through PCR, agglutination tests, and Sanger sequencing; S. Infantis (84.6%) was identified as the dominant type. The other types found included S. Enteritidis (7.7%) and S. Virchow (7.7%). Additionally, antibiotic susceptibility was tested according to EUCAST and CLSI standards in different Salmonella serotypes. The serotypes were analyzed for susceptibility to 13 antibiotics using agar-disk diffusion assays, and resistance levels were determined via E-test.
RESULTS: The disc diffusion method revealed resistance to cefazolin across all Salmonella serotypes. High resistance rates were also observed for pefloxacin (84.6%), azithromycin (76.9%), and tetracycline (84.6%). Multidrug resistance was identified in 11 (84.6%) strains by the disc diffusion test. The minimum inhibitory concentration testing with MIC test strips showed high tetracycline resistance at 84.6%. The bla TEM gene was found in 30.7% of strains, while bla CTX-M subgroup 1 (7.7%) and bla CTX-M subgroup 9 (30%) were detected by multiplex PCR; however, and bla CTX-M, bla OXA-2, and bla SHV genes were not present. Resistance to carbapenem and colistin was also checked via PCR, and bla OXA-48, bla VIM, bla NDM, bla KPC, and mcr genes were not detected in the Salmonella serotypes.
CONCLUSION: This pioneering study provides a comprehensive analysis of serotyping and ESBL production in Salmonella strains isolated from Istanbul.
METHODS: The food samples were isolated according to ISO 6579-1 and 13 (6.2%) of Salmonella strains confirmed through PCR, agglutination tests, and Sanger sequencing; S. Infantis (84.6%) was identified as the dominant type. The other types found included S. Enteritidis (7.7%) and S. Virchow (7.7%). Additionally, antibiotic susceptibility was tested according to EUCAST and CLSI standards in different Salmonella serotypes. The serotypes were analyzed for susceptibility to 13 antibiotics using agar-disk diffusion assays, and resistance levels were determined via E-test.
RESULTS: The disc diffusion method revealed resistance to cefazolin across all Salmonella serotypes. High resistance rates were also observed for pefloxacin (84.6%), azithromycin (76.9%), and tetracycline (84.6%). Multidrug resistance was identified in 11 (84.6%) strains by the disc diffusion test. The minimum inhibitory concentration testing with MIC test strips showed high tetracycline resistance at 84.6%. The bla TEM gene was found in 30.7% of strains, while bla CTX-M subgroup 1 (7.7%) and bla CTX-M subgroup 9 (30%) were detected by multiplex PCR; however, and bla CTX-M, bla OXA-2, and bla SHV genes were not present. Resistance to carbapenem and colistin was also checked via PCR, and bla OXA-48, bla VIM, bla NDM, bla KPC, and mcr genes were not detected in the Salmonella serotypes.
CONCLUSION: This pioneering study provides a comprehensive analysis of serotyping and ESBL production in Salmonella strains isolated from Istanbul.