Transactions of the Royal Society of Tropical Medicine and Hygiene · 2019
A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
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Parkhouse R Michael E, Carpio Arturo, Campoverde Alfredo, Sastre Patricia, Rojas Glenda, Harrison Leslie J S, Cortez Maria Milagros
Аннотация
BACKGROUND: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
METHODS: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
RESULTS: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
CONCLUSIONS: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.
METHODS: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
RESULTS: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
CONCLUSIONS: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.