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Войти в исследования BMC research notes · 2016
EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Wang Xiaowen, Xiao Xiangqian, Zhao Miao, Liu Wei, Pang Lin, Sun Xin, Cen Shan, Yang Burton B, Huang Yuming, Sheng Wang, Zeng Yi
Аннотация
BACKGROUND: Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection.
RESULTS: EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1-VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge.
CONCLUSIONS: Co-expression of four EV71 structural proteins VP1-VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.
RESULTS: EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1-VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge.
CONCLUSIONS: Co-expression of four EV71 structural proteins VP1-VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.
Предметные рубрики MeSH
AnimalsAnimals, NewbornAntibodies, NeutralizingAntibodies, ViralCapsid ProteinsDisease Models, AnimalEnterovirus A, HumanFemaleGene ExpressionHand, Foot and Mouth DiseaseImmunity, HumoralMiceMice, Inbred BALB CNeutralization TestsPlasmidsProtein IsoformsRecombinant ProteinsSaccharomyces cerevisiaeVaccinationVaccines, Virus-Like ParticleViral Vaccines