Chromatographic separation and isolation of bioactive compounds, 1H, 13C nuclear magnetic resonance analysis, antioxidant, antimicrobial and antiulcer potential of isolates from Olax psittacorum extract.

The herb Olax psittacorum (Lam.) Vahl. has been used traditionally for treating various ailments. The current research reports the isolation, identification, and characterisation of bioactive compounds from O. psittacorum leaf ethanolic extract and assessing their antioxidant as well as antimicrobial properties. Chromatographic separation of bioactive compounds was performed by thin layer chromatography and column chromatography, and were identified using proton (1H), carbon (13 C) Nuclear Magnetic Resonance spectroscopy along with Gas Chromatography-Mass Spectrometry analysis. Potential functional groups within the isolates were characterised by Fourier transform-infra-red spectroscopy Analysis. The isolate Pra-a demonstrated good antioxidant properties against 2,2'-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The isolated compounds exhibited good antibacterial properties with the Zone of inhibition ranging from 3 to 15 mm. The compounds exhibited good docking score as compared to the standard ofloxacin. The isolates from O. psittacorum leaf possess considerable antioxidant, antibacterial, antiulcer properties, suggesting potential pharmaceutical utilisation through purification and characterisation.