[Effect of different doses of retinol acetate on the RNA and total protein content of rodent ovaries and endometrium].

The effects of different doses of retinol acetate (RA) on protein synthesis by the hamster ovaries and endometrium were studied. Two groups of CBA/C57BL mice aged 3-4 months were given 3.44% oily RA per os on a daily basis for 10 days. After completion of the experiments the paraffin sections 6 micron m thin were subjected to a histochemical assay for the content of RNA and total protein in the ovaries and endometrium. RA (50 000 IU) was found to stimulate RNA and protein synthesis in all the ovarian structures and endometrium. In the ovaries, vitamin primarily affected the follicular epithelium and internal the-ca of the follicles. Administration of RA in a dose of 80 000 IU inhibited protein synthesis in the ovaries and endometrium. As for the ovarian structures the greatest changes under toxic hypervitaminosis A (80 000 IU) were experienced by the follicular epithelium and ovocytes.