Wei sheng yan jiu = Journal of hygiene research · 2025
[Contamination and antimicrobial resistance of Salmonella from retail aquatic products in Beijing from 2023 to 2024].
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Tian Maosong, Hu Yujie, Liu Shiwei, Li Bingyan, Li Menghan, Wu Yongning, Bai Li
Аннотация
OBJECTIVE: To understand the contamination and antimicrobial resistance of Salmonella in aquatic products in Beijing.
METHODS: A total of 216 snails and shrimps were collected from wholesale markets, wet markets and supermarkets in Beijing from July 2023 to July 2024. Broth microdilution method was used for testing the antimicrobial susceptibility of Salmonella isolates against 18 antimicrobial drugs which belong to 11 categories, and whole genome sequencing and bioinformatics analysis were performed. The association between the drug resistant phenotype and the antibiotic resistance genes(ARGs) were analyzed.
RESULTS: In total, 24 Salmonella strains were detected from 15 samples, with a detection rate of 6.9%(15/216). There was no statistically significant difference among different seasons, sampling place types, and sample sources of the detection rate. Antimicrobial susceptibility result showed that the 24 strains had the highest resistance rate to tetracycline(54.2%, 13/24), followed by trimethoprim-sulfamethoxazole(50.0%, 12/24), chloramphenicol(50.0%, 12/24) and florfenicol(41.7%, 10/24), while all strains were susceptible to imipenem. The multi-drug resistance rate was 41.7%(10/24), and some isolates could be concurrent resistant to as much as 9 classes of antimicrobials. There were 10 drug resistance spectrums, and the dominant one was TET-CHL-FFC(n=6). The 24 isolates were classified into 11 serotypes, with S. Havana(n=5) being the dominant one, followed by S. Havana. Liverpool(n=3), S. Augustenborg(n=2), S. Agona(n=2), S. Thompson(n=2), S. Telelkebir(n=2), S. Indiana(n=2), S. Kentucky(n=2), S. Corvallis(n=2), S. Corvallis(n=2), S. Potsdam(n=1) and S. Goldcoast or S. Brikama(n=1), there was a sample that detected two serotypes simultaneously. Multi-locus sequence typing(MLST) result showed that ST1527 was the main type, accounting for 20.8%(5/24). Genomic analysis showed 56 distinct ARGs in 24 Salmonella isolates, which mainly carried tet(A), floR, sul2, mphA, tet(M) and so on, and found a S. Indiana carrying the colistin resistance gene mcr-1, respectively. The relationship between ARGs and drug-resistant phenotypes found that the Kappa value of clinically important drugs, including azithromycin, cefotaxime and ciprofloxacin all beyond 0.50, the prediction result of ARGs were well consistent with the resistant phenotype.
CONCLUSION: Salmonella contamination existed in the retail link of aquatic products in Beijing, and the phenomenon of multi-drug resistance was serious, and there were many serotypes and drug resistance genes in isolates.
METHODS: A total of 216 snails and shrimps were collected from wholesale markets, wet markets and supermarkets in Beijing from July 2023 to July 2024. Broth microdilution method was used for testing the antimicrobial susceptibility of Salmonella isolates against 18 antimicrobial drugs which belong to 11 categories, and whole genome sequencing and bioinformatics analysis were performed. The association between the drug resistant phenotype and the antibiotic resistance genes(ARGs) were analyzed.
RESULTS: In total, 24 Salmonella strains were detected from 15 samples, with a detection rate of 6.9%(15/216). There was no statistically significant difference among different seasons, sampling place types, and sample sources of the detection rate. Antimicrobial susceptibility result showed that the 24 strains had the highest resistance rate to tetracycline(54.2%, 13/24), followed by trimethoprim-sulfamethoxazole(50.0%, 12/24), chloramphenicol(50.0%, 12/24) and florfenicol(41.7%, 10/24), while all strains were susceptible to imipenem. The multi-drug resistance rate was 41.7%(10/24), and some isolates could be concurrent resistant to as much as 9 classes of antimicrobials. There were 10 drug resistance spectrums, and the dominant one was TET-CHL-FFC(n=6). The 24 isolates were classified into 11 serotypes, with S. Havana(n=5) being the dominant one, followed by S. Havana. Liverpool(n=3), S. Augustenborg(n=2), S. Agona(n=2), S. Thompson(n=2), S. Telelkebir(n=2), S. Indiana(n=2), S. Kentucky(n=2), S. Corvallis(n=2), S. Corvallis(n=2), S. Potsdam(n=1) and S. Goldcoast or S. Brikama(n=1), there was a sample that detected two serotypes simultaneously. Multi-locus sequence typing(MLST) result showed that ST1527 was the main type, accounting for 20.8%(5/24). Genomic analysis showed 56 distinct ARGs in 24 Salmonella isolates, which mainly carried tet(A), floR, sul2, mphA, tet(M) and so on, and found a S. Indiana carrying the colistin resistance gene mcr-1, respectively. The relationship between ARGs and drug-resistant phenotypes found that the Kappa value of clinically important drugs, including azithromycin, cefotaxime and ciprofloxacin all beyond 0.50, the prediction result of ARGs were well consistent with the resistant phenotype.
CONCLUSION: Salmonella contamination existed in the retail link of aquatic products in Beijing, and the phenomenon of multi-drug resistance was serious, and there were many serotypes and drug resistance genes in isolates.