Pharmacological estimation of histamine in the intestine of mice experimentally infected with repeated doses of Ancylostoma caninum larvae.

Intestinal extracts from normal [uninfected] and experimental [infected with a repeated dose of 500 + 500 + 1000 Ancylostoma caninum larvae] mice were bioassayed for histamine using isolated guinea pig ileum preparation. Intestinal histamine level of experimental animals significantly increased on 5th [70 ng] and 30th [75 ng] day after the last stimulating dose of infection. The source of this excess histamine and its role in immunity have been discussed.