The IgA subclass distributions of endomysium and gliadin antibodies in human sera are different.

Subclass distributions of total IgA, IgA gliadin antibodies and IgA endomysium antibodies were investigated in 26 sera of patients with coeliac disease or dermatitis herpetiformis by means of enzyme immunoassay and fluorescence microscopy. Eight-point-three percent of total IgA belonged to the IgA2 subclass. The fraction of IgA2 gliadin antibodies amounted to 30.6% of IgA gliadin antibodies and was significantly different from the proportion of total IgA2. The relative percentage of IgA2 endomysium antibodies was very close to that of total IgA2 in serum (6.2%) and significantly lower than that of IgA2 gliadin antibodies. The results suggest an intestinal origin of gliadin antibodies. Different subclass distribution of IgA endomysium antibodies may be due to extraintestinal production or to the different nature of the endomysium antigen eliciting a more prominent IgA1 response than gliadin.