The use of an excretory-secretory antigen for an ELISA specific sero-diagnosis of visceral larva migrans.

In sera from patients with visceral larva migrans (VLM) syndrome, enzyme-linked immunospecific assay (ELISA) was used to detect IgG and IgE antibody anti-excretory-secretory antigen (ESA) from the second larval stage of Toxocara canis. The technical conditions of the assay were determined. The specificity of IgG ELISA-ESA (with OD values greater than 0.34) allowed the differentiation of VLM syndrome from ascaris or other human parasite infections.