iScience · 2022
The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.
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Calvet Charlotte, Peineau Thibault, Benamer Najate, Cornille Maxence, Lelli Andrea, Plion Baptiste, Lahlou Ghizlène, Fanchette Julia, Nouaille Sylvie, Boutet de Monvel Jacques, Estivalet Amrit, Jean Philippe, Michel Vincent, Sachse Martin, Michalski Nicolas, Avan Paul, Petit Christine, Dulon Didier, Safieddine Saaid
Аннотация
Hearing depends on fast and sustained calcium-dependent synaptic vesicle fusion at the ribbon synapses of cochlear inner hair cells (IHCs). The implication of the canonical neuronal SNARE complex in this exocytotic process has so far remained controversial. We investigated the role of SNAP-25, a key component of this complex, in hearing, by generating and analyzing a conditional knockout mouse model allowing a targeted postnatal deletion of Snap-25 in IHCs. Mice subjected to IHC Snap-25 inactivation after hearing onset developed severe to profound deafness because of defective IHC exocytosis followed by ribbon degeneration and IHC loss. Viral transfer of Snap-25 in these mutant mice rescued their hearing function by restoring IHC exocytosis and preventing synapses and hair cells from degeneration. These results demonstrate that SNAP-25 is essential for normal hearing function, most likely by ensuring IHC exocytosis and ribbon synapse maintenance.