Neural circuit underlying individual differences in visual escape habituation.

Emotions like fear help organisms respond to threats. Repeated predator exposure leads to adaptive responses with unclear neural mechanisms behind individual variability. We identify two escape behaviors in mice-persistent escape (T1) and rapid habituation (T2)-linked to unique arousal states under repetitive looming stimuli. Combining multichannel recording, circuit mapping, optogenetics, and behavioral analyses, we find parallel pathways from the superior colliculus (SC) to the basolateral amygdala (BLA) via the ventral tegmental area (VTA) for T1 and via the mediodorsal thalamus (MD) for T2. T1 involves heightened arousal, while T2 features rapid habituation. The MD integrates SC and insular cortex inputs to modulate arousal and defensive behaviors. This work reveals neural circuits underpinning adaptive threat responses and individual variability.