The Journal of clinical endocrinology and metabolism · 2018
Patients With Autoimmune Thyroiditis Show Diminished Levels and Defective Suppressive Function of Tr1 Regulatory Lymphocytes.
СтатьяНаблюдательное исследованиеПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Vitales-Noyola Marlen, Serrano-Somavilla Ana, Martínez-Hernández Rebeca, Sampedro-Nuñez Miguel, Ramos-Levi Ana M, González-Amaro Roberto, Marazuela Mónica
Аннотация
CONTEXT: T regulatory type 1 (Tr1) cells are a subpopulation of T lymphocytes (CD4+CD49+LAG-3+IL-10+) that exert a considerable immunosuppressive effect. However, their possible role in autoimmune thyroid disease (AITD) has not been explored so far.
PURPOSE: To analyze the levels and function of Tr1 cells in peripheral blood and thyroid tissue of patients with AITD.
DESIGN: Cases and controls, observational study.
SETTING: Department of Endocrinology, Hospital Universitario de la Princesa, Madrid, Spain.
PATIENTS: Thirty-eight patients with AITD (23 with Graves disease and 15 with Hashimoto thyroiditis) and 26 controls.
INTERVENTION: Multiparametric flow cytometry and immunofluorescence techniques were used to analyze the levels in peripheral blood (n = 38) and thyroid mononuclear cells (n = 5). An in vitro assay of suppression of cellular activation and cytokine release was performed to study the function of Tr1 cells.
MAIN OUTCOME MEASURE: Levels and function of Tr1 cells in patients with AITD and controls.
RESULTS: Levels of Tr1 cells were significantly diminished in peripheral blood from patients with AITD. Functional studies showed that Tr1 cells from patients with AITD exhibit a diminished suppressive function compared with healthy controls. Tr1 levels were associated with disease severity, including longer duration of the disease and ophthalmopathy activity, and with autoantibody titers.
CONCLUSIONS: The low levels of Tr1 cells and their diminished function may have a relevant role in the defective immune-regulatory function characteristic of patients with AITD.
PURPOSE: To analyze the levels and function of Tr1 cells in peripheral blood and thyroid tissue of patients with AITD.
DESIGN: Cases and controls, observational study.
SETTING: Department of Endocrinology, Hospital Universitario de la Princesa, Madrid, Spain.
PATIENTS: Thirty-eight patients with AITD (23 with Graves disease and 15 with Hashimoto thyroiditis) and 26 controls.
INTERVENTION: Multiparametric flow cytometry and immunofluorescence techniques were used to analyze the levels in peripheral blood (n = 38) and thyroid mononuclear cells (n = 5). An in vitro assay of suppression of cellular activation and cytokine release was performed to study the function of Tr1 cells.
MAIN OUTCOME MEASURE: Levels and function of Tr1 cells in patients with AITD and controls.
RESULTS: Levels of Tr1 cells were significantly diminished in peripheral blood from patients with AITD. Functional studies showed that Tr1 cells from patients with AITD exhibit a diminished suppressive function compared with healthy controls. Tr1 levels were associated with disease severity, including longer duration of the disease and ophthalmopathy activity, and with autoantibody titers.
CONCLUSIONS: The low levels of Tr1 cells and their diminished function may have a relevant role in the defective immune-regulatory function characteristic of patients with AITD.