Alimentary pharmacology & therapeutics · 2025
Patient Plasma-Induced Neutrophil Dysfunction Is Associated With Infection Risk and Mortality in Patients With Acute Decompensation of Cirrhosis.
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Sriphoosanaphan Supachaya, Macnaughtan Jane, Lu Haw, Sawhney Rohit, Zhao Qianwen, Su Lin, Gallego-Duran Rocio, Wey Emmanuel, Davies Nathan, Mookerjee Rajeshwar P, Jalan Rajiv
Аннотация
BACKGROUND AND AIMS: Bacterial infection is a major cause of morbidity and mortality in patients with acute decompensation (AD) of cirrhosis. This study aimed to investigate patient plasma-induced dysfunction of healthy neutrophils (PIND) as a predictor of subsequent infection and mortality in patients with AD.
METHODS: One hundred sixty AD patients (117 AD-NoACLF and 43 AD-ACLF), with a predominantly alcohol-related liver disease, were prospectively recruited. Plasma from 21 healthy controls and 74 patients with stable compensated cirrhosis was used as controls. Patient plasma was co-incubated with neutrophils from healthy donors to assess neutrophil function, including reactive oxygen species (ROS) production and phagocytic capacity, and CD62L surface expression.
RESULTS: Plasma from AD patients induced a primed state in healthy neutrophils, characterised by increased constitutive and N-formyl-Met-Leu-Phe (fMLP)-induced ROS production. However, effector functions were impaired, with significantly reduced phorbol 12-myristate 13-acetate (PMA)-induced ROS production, phagocytic capacity and CD62L expression. Escherichia coli- induced ROS production predicted subsequent infection in the total AD and AD-NoACLF cohorts (p = 0.043 and p = 0.013, respectively), while PMA-induced ROS production predicted 90-day mortality in the AD-NoACLF group (p = 0.041). None of the neutrophil function assays were predictive of clinical outcomes in AD-ACLF patients.
CONCLUSIONS: Plasma from patients with AD induces a 'primed but exhausted' neutrophil phenotype, characterised by a paradoxical failure to respond to stimuli. This dysfunction was associated with an increased risk of infection and mortality, particularly in AD-NoACLF patients. PIND offers a promising strategy to improve risk stratification in this high-risk population.
METHODS: One hundred sixty AD patients (117 AD-NoACLF and 43 AD-ACLF), with a predominantly alcohol-related liver disease, were prospectively recruited. Plasma from 21 healthy controls and 74 patients with stable compensated cirrhosis was used as controls. Patient plasma was co-incubated with neutrophils from healthy donors to assess neutrophil function, including reactive oxygen species (ROS) production and phagocytic capacity, and CD62L surface expression.
RESULTS: Plasma from AD patients induced a primed state in healthy neutrophils, characterised by increased constitutive and N-formyl-Met-Leu-Phe (fMLP)-induced ROS production. However, effector functions were impaired, with significantly reduced phorbol 12-myristate 13-acetate (PMA)-induced ROS production, phagocytic capacity and CD62L expression. Escherichia coli- induced ROS production predicted subsequent infection in the total AD and AD-NoACLF cohorts (p = 0.043 and p = 0.013, respectively), while PMA-induced ROS production predicted 90-day mortality in the AD-NoACLF group (p = 0.041). None of the neutrophil function assays were predictive of clinical outcomes in AD-ACLF patients.
CONCLUSIONS: Plasma from patients with AD induces a 'primed but exhausted' neutrophil phenotype, characterised by a paradoxical failure to respond to stimuli. This dysfunction was associated with an increased risk of infection and mortality, particularly in AD-NoACLF patients. PIND offers a promising strategy to improve risk stratification in this high-risk population.