Journal of infection and public health · 2025
Environmental surface sampling of respiratory and gastrointestinal pathogens during the 2022-2023 Grand Magal of Touba.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ouaddane Ihssane, Diouf Coumba, Diatta Georges, Sokhna Cheikh, Gautret Philippe
Аннотация
BACKGROUND: The Grand Magal of Touba (GMT) pilgrimage is known to be associated with risks of infection, but no studies on the role microbial contamination of the environment has been conducted yet.
MATERIALS AND METHODS: We sampled pilgrims and surfaces in the houses where they were accommodated during the 2022-2023 GMT. Respiratory and gastrointestinal pathogens were tested by qPCR.
RESULTS: Of the 152 surfaces sampled, 10.5 % tested positive for Streptococcus pneumoniae, 5.9 % for Staphylococcus aureus, 16.4 % for SARS-CoV-2 (Severe acute respiratory syndrome Coronavirus 2), 3.9 % for Klebsiella pneumoniae, and 0.6 % for rhinovirus. Only Escherichia coli was positive out of all the gastrointestinal pathogens tested, with enteropathogenic E. coli (EPEC) (23.7 %), and enteroaggregative E. coli (EAEC) (22.4 %) being the most frequent. Of 71 pilgrims tested, 66.2 % acquired a respiratory pathogen, including 16.9 % Haemophilus spp., 7.0 % SARS-CoV-2, 11.3 % Streptococcus pneumoniae, 5.6 % Staphylococcus aureus, 1.4 % rhinovirus, and 2.8 % influenza virus. A further 23.9 % acquired a gastrointestinal pathogen, including EAEC (18.3 %), EPEC (2.8 %), Giardia lamblia (5.6 %) and EIEC/Shigella (2.8 %).
CONCLUSION: These preliminary results showed a good correlation between results of surface sampling and human infection for SARS-CoV-2 and EAEC. SARS-CoV-2 surface sampling could be used as a surveillance tool during the GMT. Further larger-scale studies are needed to confirm these preliminary findings.
MATERIALS AND METHODS: We sampled pilgrims and surfaces in the houses where they were accommodated during the 2022-2023 GMT. Respiratory and gastrointestinal pathogens were tested by qPCR.
RESULTS: Of the 152 surfaces sampled, 10.5 % tested positive for Streptococcus pneumoniae, 5.9 % for Staphylococcus aureus, 16.4 % for SARS-CoV-2 (Severe acute respiratory syndrome Coronavirus 2), 3.9 % for Klebsiella pneumoniae, and 0.6 % for rhinovirus. Only Escherichia coli was positive out of all the gastrointestinal pathogens tested, with enteropathogenic E. coli (EPEC) (23.7 %), and enteroaggregative E. coli (EAEC) (22.4 %) being the most frequent. Of 71 pilgrims tested, 66.2 % acquired a respiratory pathogen, including 16.9 % Haemophilus spp., 7.0 % SARS-CoV-2, 11.3 % Streptococcus pneumoniae, 5.6 % Staphylococcus aureus, 1.4 % rhinovirus, and 2.8 % influenza virus. A further 23.9 % acquired a gastrointestinal pathogen, including EAEC (18.3 %), EPEC (2.8 %), Giardia lamblia (5.6 %) and EIEC/Shigella (2.8 %).
CONCLUSION: These preliminary results showed a good correlation between results of surface sampling and human infection for SARS-CoV-2 and EAEC. SARS-CoV-2 surface sampling could be used as a surveillance tool during the GMT. Further larger-scale studies are needed to confirm these preliminary findings.