Effect of Three Different Topical Fluoride Varnishes on Streptococcus mutans Count in Biofilm Samples of Children Aged 6-10 Years: A Randomized Controlled Trial.

OBJECTIVE

This study aims to check how well three fluoride varnishes work. The varnishes are GC MI Varnish, Ultradent Enamelast, and Ivoclar Fluor Protector. They are tested to see if they can lower Streptococcus mutans levels in biofilm samples from children aged 6-10 years.

MATERIALS AND

METHODS

A randomized controlled trial was conducted with 144 children divided into three groups. Samples of plaque were gathered initially and then again at intervals of 1, 3, and 6 months following the application of fluoride varnish. The levels of S. mutans were measured using culture methods and reported as colony-forming units per milliliter (CFU/mL).

RESULTS

All three fluoride varnishes significantly reduced S. mutans counts over the 6-month period. GC MI Varnish showed the greatest reduction, particularly at 1 and 3 months (p < 0.0001 and p = 0.01, respectively). The mean CFU/mL for GC MI Varnish decreased from 7.64 ± 0.18 at baseline to 5.76 ± 2.05 at 6 months. Ultradent Enamelast and Fluor Protector S also demonstrated significant reductions but to a lesser extent than GC MI Varnish.

CONCLUSION

GC MI Varnish containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) demonstrated superior antimicrobial efficacy against S. mutans compared to Ultradent Enamelast and Fluor Protector S. All three fluoride varnishes were effective in reducing S. mutans counts, with GC MI Varnish showing the most significant and consistent reductions over time.

HOW TO CITE THIS ARTICLE: Baidya D, Pathivada L, Garg N, et al. Effect of Three Different Topical Fluoride Varnishes on Streptococcus mutans Count in Biofilm Samples of Children Aged 6-10 Years: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2026;19(3):343-349.