[Expression of Th17 related cell factors and its significance in CRSsNP].

OBJECTIVE

To detect the expression of Th17 nuclear factor RORC and cytokines IL-17A and IL-22 and neutrophil marker MPO and their correlations with CRSsNP.

METHOD: RT-PCR was used to detect mRNA expression of RORC, IL-17A and IL-22. Immunohistochemistry was used to assess the IL-17A positive cells in CRSsNP and control. ELISA was used to detect the expression of MPO.

RESULT: CRSsNP had higher mRNA expression of RORC, IL-17A and IL-22 and increased protein expression of MPO. The mRNA expression of RORC, IL-17A and IL-22 was positively correlated with each other but none of them was correlated with the expression of MPO in CRSsNP and control.

CONCLUSION

Both Th17 and neutrophils contribute to the pathogenesis of CRSsNP, however, the neutrophil infiltration may not be recruited by Th17 cytokines.