Journal of applied microbiology · 2025
Klebsiella pneumoniae complex isolated from diseased companion animals reveals genomic diversity, multidrug resistance, and extended-spectrum β-lactamase-encoding genes.
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de Paula Carolina Lechinski, Rissetti Rafaela Mastrangelo, Yamada Amanda Yaeko, Bertani Amanda Maria de Jesus, Sacchi Claudio Tavares, Campos Karoline Rodrigues, Portilho Fábio Vinícius Ramos, Paz Patrik Júnior de Lima, Bello Thaís Spessotto, Arabe Filho Marcelo Fagali, Panegossi Letícia Colin, Reznik Alec Utida, Ribeiro Márcio Garcia, Camargo Carlos Henrique
Аннотация
AIMS: To evaluate the antimicrobial susceptibility and clonal relatedness of Klebsiella pneumoniae isolates recovered from diseased companion animals at a Teaching Veterinary Hospital in Brazil.
METHODS AND RESULTS: A collection of 59 K. pneumoniae complex isolates originating from dogs and cats were subjected to antimicrobial susceptibility testing, multiple antimicrobial resistance (MAR) index determination, polymerase chain reaction for carbapenemase-encoding genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Nineteen isolates were subjected to whole-genome sequencing (WGS). MAR indices ranged from 0 to 0.73, 50 different PFGE restriction profiles were identified. No carbapenemase genes were detected. Seventeen different sequence types were identified among the 19 sequenced strains, and extended-spectrum β-lactamase genes were detected in five of them.
CONCLUSIONS: Klebsiella pneumoniae isolates from companion animals revealed high genetic diversity, both in PFGE and multi-locus sequence typing analysis; conversely, they presented resistance genes commonly found in human clinical isolates.
METHODS AND RESULTS: A collection of 59 K. pneumoniae complex isolates originating from dogs and cats were subjected to antimicrobial susceptibility testing, multiple antimicrobial resistance (MAR) index determination, polymerase chain reaction for carbapenemase-encoding genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Nineteen isolates were subjected to whole-genome sequencing (WGS). MAR indices ranged from 0 to 0.73, 50 different PFGE restriction profiles were identified. No carbapenemase genes were detected. Seventeen different sequence types were identified among the 19 sequenced strains, and extended-spectrum β-lactamase genes were detected in five of them.
CONCLUSIONS: Klebsiella pneumoniae isolates from companion animals revealed high genetic diversity, both in PFGE and multi-locus sequence typing analysis; conversely, they presented resistance genes commonly found in human clinical isolates.
Предметные рубрики MeSH
AnimalsKlebsiella pneumoniaebeta-LactamasesDrug Resistance, Multiple, BacterialKlebsiella InfectionsCatsDogsMicrobial Sensitivity TestsGenetic VariationElectrophoresis, Gel, Pulsed-FieldBrazilAnti-Bacterial AgentsPetsMultilocus Sequence TypingWhole Genome SequencingDog DiseasesCat DiseasesBacterial Proteins