Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2026
Alleviation of nitroglycerin-induced migraine in rats: inhibition of central sensitization by optimizing Qinggan Jieyu decoction regulation of purinergic receptor P2X ligand-gated ion channel 7 and autophagy.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Jiazheng L I, Bo Zhou
Аннотация
OBJECTIVE: To investigate the effects of optimizing Qinggan Jieyu decoction on purinergic receptor P2X ligand-gated ion channel 7 (P2X7R) and autophagy in migraine model rats based on molecular biology and histopathology.
METHODS: A migraine rat model was established by a single subcutaneous nitroglycerin (NTG) injection into the posterior neck. QGJY was administered via gavage for 7 d prior to NTG induction. Behavioral changes, central sensitization biomarkers, and inflammatory cytokine levels were analyzed to evaluate migraine severity. Western blot, immunofluorescence, quantitative real-time PCR, and transmission electron microscopy were employed to assess P2X7R expression and autophagy activity in trigeminal nucleus caudalis (TNC) tissues. The P2X7R agonist 2' (3')-O-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP) was further utilized to validate QGJY's regulatory effects.
RESULTS: QGJY significantly reduced cage-climbing and head-scratching frequencies in NTG-induced migraine rats, downregulated serum and TNC levels of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha, and suppressed central sensitization markers (substance P; calcitonin gene-related peptide; and c-fos induced growth factor) in TNC tissues (P < 0.05). QGJY markedly decreased microglial cell counts and average immunofluorescence intensity in TNC tissues and promoted elongation of microglial protrusions (P < 0.05). Concurrently, QGJY downregulated P2X7R protein and mRNA expression, reduced the light chain 3 (LC3)-II/LC3-I ratio, elevated ubiquitin-binding protein p62 levels, and diminished autophagosome numbers in TNC tissues (P < 0.05). Furthermore, QGJY reversed BzATP-induced P2X7R upregulation (P < 0.05).
CONCLUSIONS: QGJY alleviates migraine and inhibits central sensitization in rats, potentially by downregulating P2X7R expression, concomitantly suppressing autophagy, attenuating microglial activation, and reducing pro-inflammatory cytokine release.
METHODS: A migraine rat model was established by a single subcutaneous nitroglycerin (NTG) injection into the posterior neck. QGJY was administered via gavage for 7 d prior to NTG induction. Behavioral changes, central sensitization biomarkers, and inflammatory cytokine levels were analyzed to evaluate migraine severity. Western blot, immunofluorescence, quantitative real-time PCR, and transmission electron microscopy were employed to assess P2X7R expression and autophagy activity in trigeminal nucleus caudalis (TNC) tissues. The P2X7R agonist 2' (3')-O-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP) was further utilized to validate QGJY's regulatory effects.
RESULTS: QGJY significantly reduced cage-climbing and head-scratching frequencies in NTG-induced migraine rats, downregulated serum and TNC levels of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha, and suppressed central sensitization markers (substance P; calcitonin gene-related peptide; and c-fos induced growth factor) in TNC tissues (P < 0.05). QGJY markedly decreased microglial cell counts and average immunofluorescence intensity in TNC tissues and promoted elongation of microglial protrusions (P < 0.05). Concurrently, QGJY downregulated P2X7R protein and mRNA expression, reduced the light chain 3 (LC3)-II/LC3-I ratio, elevated ubiquitin-binding protein p62 levels, and diminished autophagosome numbers in TNC tissues (P < 0.05). Furthermore, QGJY reversed BzATP-induced P2X7R upregulation (P < 0.05).
CONCLUSIONS: QGJY alleviates migraine and inhibits central sensitization in rats, potentially by downregulating P2X7R expression, concomitantly suppressing autophagy, attenuating microglial activation, and reducing pro-inflammatory cytokine release.