A domestic cell bioreactor and its application in virus culture.

A cell culture bioreactor (CellCul-20) and its application in cell and virus culture are described in this paper. It has been evaluated with strict aseptic tests and one-year's operation shown that CellCul-20 bioreactor can keep its aseptic condition after being autoclaved. It can meet the requirement for the control of the main parameters for cell and virus culture and the finely adjustment of the main parameters to meet the changing conditions of the cultivation. A high cell density and a high level of virus titre were reached respectively for Vero cells and Japanese encephalitis virus (JEV) while they were cultured in this bioreactor. It is the first report on large-scale culture of JEV-infected Vero cells to prepare primary JEV vaccine. Some suggestions are made for the improvement of CellCul-20.