Simple and Efficient In Vitro Fertilization and Embryo Transfer in Mice.

Mice, as one of the most important model organisms in life science research, rely heavily on assisted reproductive technologies, particularly in vitro fertilization (IVF) and embryo transfer (ET), which serve as indispensable platforms for genetic modification, strain preservation and expansion, as well as studies in reproductive biology. This article provides a detailed description of an optimized and validated experimental system encompassing the entire workflow from superovulation, sperm collection and capacitation, in vitro fertilization, embryo culture, and vasectomy of male mice, to embryo transfer and related auxiliary procedures. © 2026 Wiley Periodicals LLC. Basic Protocol 1: IVF in mice Basic Protocol 2: Oviduct ET in mice Support Protocol: Preparation of vasectomized males and pseudopregnant females.