Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026
[Total Astragalus saponins reduce astrocyte swelling and AQP4 expression following oxygen-glucose deprivation and reoxygenation by inhibiting the p38 pathway].
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Yin Qin, Lei Yuxiao, Wang Xiaoshu, Yang Gang, Tang Zhaohua
Аннотация
OBJECTIVES: To investigate the effect of total Astragalus saponins (AST) on astrocyte swelling induced by glucose-oxygen deprivation and reoxygenation (OGD/R) and its molecular mechanism.
METHODS: Primary astrocytes isolated from the brain of post-natal day 2 SD rats were treated with solvent vehicle, AST, or AST combined with U-46619 (a p38 activator) 24 h prior to and during exposure to OGD/R. The changes in cell volume and morphology were observed, and intracellular reactive oxygen species (ROS) levels and lactate dehydrogenase (LDH) activity were detected. The expression of aquaporin 4 (AQP4) on cell membrane was observed using immunofluorescence confocal microscopy, and Western blotting was performed to detect the changes in p38 signaling pathway phosphorylation levels and AQP4 expression level.
RESULTS: AST treatment significantly reduced OGD/R-induced increase in astrocyte volume, ROS levels and LDH release. The AST-treated cells also exhibited improved cell morphology, reduced aggregation of AQP4 on the cell membrane, and decreased p38 phosphorylation and AQP4 expression levels. The protective effects of AST against OGD/R-induced cell injuries were significantly attenuated by combined treatment of the cells with U-46619.
CONCLUSIONS: AST alleviate astrocyte swelling induced by OGD/R injury by inhibiting the activation of the p38 signaling pathway and AQP4 overexpression on the cell membrane.
METHODS: Primary astrocytes isolated from the brain of post-natal day 2 SD rats were treated with solvent vehicle, AST, or AST combined with U-46619 (a p38 activator) 24 h prior to and during exposure to OGD/R. The changes in cell volume and morphology were observed, and intracellular reactive oxygen species (ROS) levels and lactate dehydrogenase (LDH) activity were detected. The expression of aquaporin 4 (AQP4) on cell membrane was observed using immunofluorescence confocal microscopy, and Western blotting was performed to detect the changes in p38 signaling pathway phosphorylation levels and AQP4 expression level.
RESULTS: AST treatment significantly reduced OGD/R-induced increase in astrocyte volume, ROS levels and LDH release. The AST-treated cells also exhibited improved cell morphology, reduced aggregation of AQP4 on the cell membrane, and decreased p38 phosphorylation and AQP4 expression levels. The protective effects of AST against OGD/R-induced cell injuries were significantly attenuated by combined treatment of the cells with U-46619.
CONCLUSIONS: AST alleviate astrocyte swelling induced by OGD/R injury by inhibiting the activation of the p38 signaling pathway and AQP4 overexpression on the cell membrane.