The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026
The Integrative and Conjugative Element ICEPmiW2 in Proteus mirabilis W2 Facilitates the Dissemination of Antibiotic-Resistance Genes.
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Cao Yahan, Yu Wenchao
Аннотация
BACKGROUND: The extensive use of antibiotics for treating infectious diseases leads to their release into the environment, which in turn results in antibiotic pollution and thereby facilitates the dissemination of antibiotic-resistance genes (ARGs). Recently, despite the implementation of strict antibiotic usage restrictions, the accumulation of ARGs and multidrug-resistant bacteria in the aquaculture environment continues to show a trend of persistent spread.
METHODS: The W2 strain was isolated in the presence of 32 μg/mL doxycycline. A broth microdilution assay was employed to determine the minimum inhibitory concentrations. Whole-genome sequencing was conducted to characterize ARGs and their mobility through bioinformatics analysis. The spread of ARGs was detected by conjugation assays.
RESULTS: W2 strain was isolated from the wastewater of a crucian carp aquaculture plant in Jinan, China, and identified as Proteus mirabilis. W2 genome contained a 190,320 bp antibiotic-resistance-conferring integrative and conjugative element (ICE), named ICEPmiW2. ICEPmiW2 contains 21 ARGs, 14 conjugative transposon protein-encoding genes, and one complex type I integron. No transconjugants were obtained using W2 as the donor strain and Escherichia coli 25DN as the recipient strain. However, evolutionary analysis revealed that ICEPmiW2 likely evolved from ICEs of other P. mirabilis strains.
CONCLUSIONS: The multiple-antibiotic-resistant P. mirabilis W2 strain with potential pathogenicity to aquatic animals was isolated, and the antibiotic-resistance-conferring ICEPmiW2 was identified in P. mirabilis W2. Our findings suggest that ICEPmiW2 of P. mirabilis W2 can potentially spread ARGs among environmental P. mirabilis strains.
METHODS: The W2 strain was isolated in the presence of 32 μg/mL doxycycline. A broth microdilution assay was employed to determine the minimum inhibitory concentrations. Whole-genome sequencing was conducted to characterize ARGs and their mobility through bioinformatics analysis. The spread of ARGs was detected by conjugation assays.
RESULTS: W2 strain was isolated from the wastewater of a crucian carp aquaculture plant in Jinan, China, and identified as Proteus mirabilis. W2 genome contained a 190,320 bp antibiotic-resistance-conferring integrative and conjugative element (ICE), named ICEPmiW2. ICEPmiW2 contains 21 ARGs, 14 conjugative transposon protein-encoding genes, and one complex type I integron. No transconjugants were obtained using W2 as the donor strain and Escherichia coli 25DN as the recipient strain. However, evolutionary analysis revealed that ICEPmiW2 likely evolved from ICEs of other P. mirabilis strains.
CONCLUSIONS: The multiple-antibiotic-resistant P. mirabilis W2 strain with potential pathogenicity to aquatic animals was isolated, and the antibiotic-resistance-conferring ICEPmiW2 was identified in P. mirabilis W2. Our findings suggest that ICEPmiW2 of P. mirabilis W2 can potentially spread ARGs among environmental P. mirabilis strains.