Journal of cardiovascular and thoracic research · 2013
Molecular diagnosis of bacterial infective endocarditis in tabriz, azerbaijan.
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Ghotaslou Reza, Salahi Eshlaghi Behnaz, Yeganeh Fatemeh, Yaghoubi Alireza
Аннотация
INTRODUCTION: The aim of this study was to analyze a PCR based approach for detection of infective endocarditis in Azerbaijan.
METHODS: Ten aortic valves, 8 mitral valves and 2 tricuspid valves, were analyzed for the presence of bacterial infective endocarditis using Gram staining, culture and PCR methods.
RESULTS: Of the 20 valves, 5 and 4 cases were positive by Gram staining and culture assay, respectively. Bacterial DNA was positive in 12 of the 20 valves (60%) by broad-spectrum PCR. Direct sequencing for species identification was possible in 10 cases.
CONCLUSION: PCR and direct molecular identification of the etiological agents responsible for infective endocarditis may enable specific treatment to begin at an earlier phase of the disease.
METHODS: Ten aortic valves, 8 mitral valves and 2 tricuspid valves, were analyzed for the presence of bacterial infective endocarditis using Gram staining, culture and PCR methods.
RESULTS: Of the 20 valves, 5 and 4 cases were positive by Gram staining and culture assay, respectively. Bacterial DNA was positive in 12 of the 20 valves (60%) by broad-spectrum PCR. Direct sequencing for species identification was possible in 10 cases.
CONCLUSION: PCR and direct molecular identification of the etiological agents responsible for infective endocarditis may enable specific treatment to begin at an earlier phase of the disease.