Differential labelling of primary olfactory system subcomponents by SBA (lectin) and NADPH-d histochemistry in the frog Pipa.

SBA and NADPH-d histochemistries allow identification of functionally distinct components of the amphibian primary olfactory system. In Pipa, a secondarily aquatic frog, combination of both methodologies, using alternate sets of histological sections, reveals that, apart from Jacobson's organ, this species has a "water-nose" and an "air-nose". The epithelia occupy separate chambers of the olfactory organ and give rise to olfactory nerve fiber bundles that are identified by the dual staining procedure.