Journal of cancer research and clinical oncology · 2008
Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials.
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Shi Guo-Ming, Xu Yang, Fan Jia, Zhou Jian, Yang Xin-Rong, Qiu Shuang-Jian, Liao Yong, Wu Wei-Zhong, Ji Yuan, Ke Ai-Wu, Ding Zhen-Bin, He Yi-Zhou, Wu Bing, Yang Guo-Huan, Qin Wen-Zhen, Zhang Wu, Zhu Jiang, Min Zhi-Hui, Wu Zhi-Quan
Аннотация
PURPOSE: To identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials.
METHODS: SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG(2) in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively.
RESULTS: There was significant difference in SP proportion among HCCLM3, MHCC97-H, MHCC97-L and Hep3B (28.7 +/- 1.6%, 14.5 +/- 0.6%, 4.2 +/- 0.4%, 0.9 +/- 0.1%, respectively, P < 0.01). All the SP cells showed similar characteristics of self-renewal, high clonogenicity, remarkable chemo-resistance and high expression of ABCG(2). As low as 2,000 SP cells could initiate tumors in non-obese diabetic/severe combined immunodeficiency mice successfully.
CONCLUSIONS: SP cells purified from HCC cell lines harbors cancer stem cell-like properties, and may be related to the metastatic potentials and therapeutic-resistance of HCC.
METHODS: SP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG(2) in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively.
RESULTS: There was significant difference in SP proportion among HCCLM3, MHCC97-H, MHCC97-L and Hep3B (28.7 +/- 1.6%, 14.5 +/- 0.6%, 4.2 +/- 0.4%, 0.9 +/- 0.1%, respectively, P < 0.01). All the SP cells showed similar characteristics of self-renewal, high clonogenicity, remarkable chemo-resistance and high expression of ABCG(2). As low as 2,000 SP cells could initiate tumors in non-obese diabetic/severe combined immunodeficiency mice successfully.
CONCLUSIONS: SP cells purified from HCC cell lines harbors cancer stem cell-like properties, and may be related to the metastatic potentials and therapeutic-resistance of HCC.
Предметные рубрики MeSH
ATP Binding Cassette Transporter, Subfamily G, Member 2ATP-Binding Cassette TransportersCarcinoma, HepatocellularCell DifferentiationCell DivisionCell Line, TumorDrug Resistance, NeoplasmFlow CytometryGene Expression Regulation, NeoplasticHumansLiver NeoplasmsNeoplasm MetastasisNeoplasm ProteinsPhenotypePrognosisSurvival RateTumor Stem Cell Assay