Zhonghua er bi yan hou ke za zhi · 1998
[Isolation of and intracellular calcium mobilization in vestibular hair cells of the patients with acoustic neuroma].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chen L, Jiang S, Yang W
Аннотация
OBJECTIVE: To further understand the biological features of the human vestibular organ.
METHODS: Vestibular hair cells were isolated from patients with acoustic neuroma during the surgery and intracellular free calcium concentration of the cells was monitored with calcium-sensitive dye Fura-2 and digital imaging microscopy.
RESULTS: Isolated vestibular hair cells were mainly of type I and could survive about 2.5 hours in vitro. Perfusion with 150 mmol/L KCl, induced a depolarization, resulting in a temporary increase of 340 nm/380 nm ratio from 0.52 to 1.16 within 1 min. In Ca2+ free environment, 150 mmol/L KCl failed to induce intracellular calcium increase. In the presence of 2.5 mumol/L ionomycin, there was an irreversible increase in 340 nm/380 nm ratio.
CONCLUSION: Human vestibular hair cell apparently possess voltage sensitive calcium channels. The method and observation aid in elucidating human vestibular biological features.
METHODS: Vestibular hair cells were isolated from patients with acoustic neuroma during the surgery and intracellular free calcium concentration of the cells was monitored with calcium-sensitive dye Fura-2 and digital imaging microscopy.
RESULTS: Isolated vestibular hair cells were mainly of type I and could survive about 2.5 hours in vitro. Perfusion with 150 mmol/L KCl, induced a depolarization, resulting in a temporary increase of 340 nm/380 nm ratio from 0.52 to 1.16 within 1 min. In Ca2+ free environment, 150 mmol/L KCl failed to induce intracellular calcium increase. In the presence of 2.5 mumol/L ionomycin, there was an irreversible increase in 340 nm/380 nm ratio.
CONCLUSION: Human vestibular hair cell apparently possess voltage sensitive calcium channels. The method and observation aid in elucidating human vestibular biological features.