Optometry and vision science : official publication of the American Academy of Optometry · 1998
Interactions of bacteria with contact lenses: the effect of soluble protein and carbohydrate on bacterial adhesion to contact lenses.
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Williams T J, Willcox M D, Schneider R P
Аннотация
PURPOSE: During contact lens wear, components in the tear film adsorb to the contact lens surface to form a protein-rich coating. In this study, we investigated the importance of these components in bacterial adhesion to contact lenses.
METHODS: Five strains of bacteria were tested for their ability to adhere to contact lenses (patient-worn; coated with lactoferrin, lysozyme, or an artificial tear formulation; or left uncoated) in the presence of various solutes (protein, carbohydrate). Bacterial adhesion was quantified by both a total count (light microscopy) and viable count (culturing on agar).
RESULTS: None of the solutes were found to competitively inhibit bacterial adhesion to worn contact lenses. However, lactoferrin was observed to kill bacteria attached to worn contact lenses.
CONCLUSIONS: We could find no evidence to indicate that bacterial adhesion to contact lenses is mediated by specific interactions between bacteria and contact lens-bound tear components. The interaction between attached bacteria and lactoferrin may be important in initiating inflammatory responses to contact lens wear.
METHODS: Five strains of bacteria were tested for their ability to adhere to contact lenses (patient-worn; coated with lactoferrin, lysozyme, or an artificial tear formulation; or left uncoated) in the presence of various solutes (protein, carbohydrate). Bacterial adhesion was quantified by both a total count (light microscopy) and viable count (culturing on agar).
RESULTS: None of the solutes were found to competitively inhibit bacterial adhesion to worn contact lenses. However, lactoferrin was observed to kill bacteria attached to worn contact lenses.
CONCLUSIONS: We could find no evidence to indicate that bacterial adhesion to contact lenses is mediated by specific interactions between bacteria and contact lens-bound tear components. The interaction between attached bacteria and lactoferrin may be important in initiating inflammatory responses to contact lens wear.