[Morphofunctional characteristics of the colon in mice Balb/c in remote terms after splenectomy].

BACKGROUND AND AIMS: Hypersusceptibility to pathogens, including enterobacteria is the most serious late result of splenectomy. The aim of these studies was to give the morphofunctional characteristics of large bowel and to analyze luminal bacterial ecosystem disturbances as late results of splenectomy.

METHODS

Fourty male mice Balb/c with body mass 18-20 g were divided in random manner: control group consisted of ten intact mice, 15 mice in each of two experimental groups subjected to splenectomy. All animal were carried in standard housing conditions and feeding and were upset of the experiment on the 30th (experimental group I) and 60th (experimental group II) days after the splenectomy. For the histological investigation the pieces of colon were taken and processed in standard histological methods. The blood out of cardial cavity was sampled for estimation of the endotoxin level; faeces for the analyzing of luminal bacterial ecosystem disturbances (dysbios) were taken out of the rectum.

RESULTS

At the 30th day after splenectomy the pattern of chronic catarrhal colitis was at the histological the stroma by lymphocytes and neutrophils. At the 60th day at the histological slides the increasing of mitotic activity of epithelial cells in crypts of the colon and activation of the inflammative infiltration were observed. The endotoxin level half as large again in comparison with the control one at the 30th day. The I-II stages of dysbiotic disturbances were recognizes by bacterial methods at the 30th day with the following development up the II stage at the 60th day.

CONCLUSIONS

The chronic catarrhal colitis with accompanying disturbance of luminal bacterial ecosystem (dysbiotic state of Stages I-II) and increasing og the endotoxin level in the blood are among other late results of splenectomy in mice Balb/c model