Neuron · 2004
The transcription factor Bhlhb4 is required for rod bipolar cell maturation.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bramblett Debra E, Pennesi Mark E, Wu Samuel M, Tsai Ming-Jer
Аннотация
Retinal bipolar cells are essential to the transmission of light information. Although bipolar cell dysfunction can result in blindness, little is known about the factors required for bipolar cell development and functional maturation. The basic helix-loop-helix (bHLH) transcription factor Bhlhb4 was found to be expressed in rod bipolar cells (RB). Electroretinograms (ERGs) in the adult Bhlhb4 knockout (Bhlhb4(-/-)) showed that the loss of Bhlhb4 resulted in disrupted rod signaling and profound retinal dysfunction resembling human congenital stationary night blindness (CSNB), characterized by the loss of the scotopic ERG b-wave. A depletion of inner nuclear layer (INL) cells in the adult Bhlhb4 knockout has been ascribed to the abolishment of the RB cell population during postnatal development. Other retinal cell populations including photoreceptors were unaltered. The timing of RB cell depletion in the Bhlhb4(-/-) mouse suggests that Bhlhb4 is essential for RB cell maturation.
Предметные рубрики MeSH
Age FactorsAnimalsAnimals, NewbornBasic Helix-Loop-Helix ProteinsBlotting, SouthernCalbindin 2CalbindinsCalcium-Binding ProteinsCaspase 3CaspasesCell CountCells, CulturedCellular SenescenceElectroretinographyEmbryo, MammalianEvoked PotentialsEye ProteinsFluorescent Antibody TechniqueGTP-Binding Protein alpha Subunits, Gi-GoGene ExpressionGene Expression Regulation, DevelopmentalHomeodomain ProteinsIn Situ HybridizationIndenesMembrane GlycoproteinsMembrane Transport ProteinsMiceMice, KnockoutMicroscopy, ElectronNerve Tissue ProteinsNeuronsPAX6 Transcription FactorPaired Box Transcription FactorsPhotic StimulationProtein Kinase CProtein Kinase C-alphaRNA, MessengerRepressor ProteinsRetinaRetinal Rod Photoreceptor CellsReverse Transcriptase Polymerase Chain ReactionS100 Calcium Binding Protein GS100 ProteinsStem CellsSynapsesTranscription FactorsVesicular Glutamate Transport Protein 1Vesicular Transport Proteinsbeta-Galactosidase