Respiratory research · 2014
Food allergy enhances allergic asthma in mice.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Bihouée Tiphaine, Bouchaud Gregory, Chesné Julie, Lair David, Rolland-Debord Camille, Braza Faouzi, Cheminant Marie-Aude, Aubert Philippe, Mahay Guillaume, Sagan Christine, Neunlist Michel, Brouard Sophie, Bodinier Marie, Magnan Antoine
Аннотация
BACKGROUND: Atopic march refers to the typical transition from a food allergy in early childhood to allergic asthma in older children and adults. However the precise interplay of events involving gut, skin and pulmonary inflammation in this process is not completely understood.
OBJECTIVES: To develop a mouse model of mixed food and respiratory allergy mimicking the atopic march and better understand the impact of food allergies on asthma.
METHODS: Food allergy to ovalbumin (OVA) was induced through intra-peritoneal sensitization and intra-gastric challenge, and/or a respiratory allergy to house dust mite (HDM) was obtained through percutaneous sensitization and intra-nasal challenges with dermatophagoides farinae (Der f) extract. Digestive, respiratory and systemic parameters were analyzed.
RESULTS: OVA-mediated gut allergy was associated with an increase in jejunum permeability, and a worsening of Der f-induced asthma with stronger airway hyperresponsiveness and pulmonary cell infiltration, notably eosinophils. There was overproduction of the pro-eosinophil chemokine RANTES in broncho-alveolar lavages associated with an enhanced Th2 cytokine secretion and increased total and Der f-specific IgE when the two allergies were present. Both AHR and lung inflammation increased after a second pulmonary challenge.
CONCLUSION: Gut sensitization to OVA amplifies Der f-induced asthma in mice.
OBJECTIVES: To develop a mouse model of mixed food and respiratory allergy mimicking the atopic march and better understand the impact of food allergies on asthma.
METHODS: Food allergy to ovalbumin (OVA) was induced through intra-peritoneal sensitization and intra-gastric challenge, and/or a respiratory allergy to house dust mite (HDM) was obtained through percutaneous sensitization and intra-nasal challenges with dermatophagoides farinae (Der f) extract. Digestive, respiratory and systemic parameters were analyzed.
RESULTS: OVA-mediated gut allergy was associated with an increase in jejunum permeability, and a worsening of Der f-induced asthma with stronger airway hyperresponsiveness and pulmonary cell infiltration, notably eosinophils. There was overproduction of the pro-eosinophil chemokine RANTES in broncho-alveolar lavages associated with an enhanced Th2 cytokine secretion and increased total and Der f-specific IgE when the two allergies were present. Both AHR and lung inflammation increased after a second pulmonary challenge.
CONCLUSION: Gut sensitization to OVA amplifies Der f-induced asthma in mice.
Предметные рубрики MeSH
AnimalsAntigens, DermatophagoidesArthropod ProteinsAsthmaBronchial HyperreactivityBronchoconstrictionChemokine CCL5Disease Models, AnimalFemaleFood HypersensitivityImmunoglobulin EIntestinal MucosaIntestinesLungMice, Inbred BALB COvalbuminPermeabilityPneumoniaPulmonary EosinophiliaTh2 CellsTime Factors