Immunobiology · 2025
Increase CD24+CD27+ B cells within pleural effusions derived from lung adenocarcinoma presents enhanced potential for clinical utility.
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Liang Yueming, Sun Danqi, Xu Qizhi, Mao Xiaofan, Li Minjing, Gao XingLin, Yu Sifei
Аннотация
BACKGROUND: The increasing interest in the roles of B cells, particularly regulatory B cells, within the tumor microenvironment has become prominent, though their immunological characteristics in malignant pleural effusions (PE) remain poorly elucidated.
METHODS: Flow cytometry was performed in 143 pleural effusion and peripheral blood samples from patients in order to analyze the proportions ofPB-derived B and T cells, and to assess surface markers and cytokines, such as IFN-γ and IL-10. Moreover, we compared clinical role of CD24+CD27+B cell populations.
RESULTS: B cell populations were significantly higher in PE from lung adenocarcinoma (LUAD)than tuberculosis (TB). Compared to PB from patients, the proportion of CD24+CD27+ B cells was markedly elevated in LUAD-PE and exhibited reduced levels of CD38, CD5, CD71,PD-1, and PD-L1, as well as an upregulation of IL-10 and CD39. PD-1 and PD-L1 werelargely found to be upregulated within the CD27+CD38+ B cell subset despite declining proportions overall. Re-interpretations illustrated significant relationshipsbetween CD24+CD27+ B cells and clinical measurements.
CONCLUSIONS: This study highlights the heterogenicphenotypes and functions of various B cell subsets in LUAD-PE, with specific attention to CD24+CD27+ B cells as potential diagnostic markers and the need for further studies investigating their immunoregulatory functions to unveil new immunotherapeutic strategies in lung cancer.
METHODS: Flow cytometry was performed in 143 pleural effusion and peripheral blood samples from patients in order to analyze the proportions ofPB-derived B and T cells, and to assess surface markers and cytokines, such as IFN-γ and IL-10. Moreover, we compared clinical role of CD24+CD27+B cell populations.
RESULTS: B cell populations were significantly higher in PE from lung adenocarcinoma (LUAD)than tuberculosis (TB). Compared to PB from patients, the proportion of CD24+CD27+ B cells was markedly elevated in LUAD-PE and exhibited reduced levels of CD38, CD5, CD71,PD-1, and PD-L1, as well as an upregulation of IL-10 and CD39. PD-1 and PD-L1 werelargely found to be upregulated within the CD27+CD38+ B cell subset despite declining proportions overall. Re-interpretations illustrated significant relationshipsbetween CD24+CD27+ B cells and clinical measurements.
CONCLUSIONS: This study highlights the heterogenicphenotypes and functions of various B cell subsets in LUAD-PE, with specific attention to CD24+CD27+ B cells as potential diagnostic markers and the need for further studies investigating their immunoregulatory functions to unveil new immunotherapeutic strategies in lung cancer.