Journal of dermatological science · 2005
Rapid and specific identification of Sporothrix schenckii by PCR targeting the DNA topoisomerase II gene.
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Kanbe Toshio, Natsume Luna, Goto Itsuko, Kawasaki Masako, Mochizuki Takashi, Ishizaki Hiroshi, Kikuchi Akihiko
Аннотация
BACKGROUND: In order to study the genotype variation of Sporothrix schenckii, we previously analyzed the genomic sequences of the DNA topoisomerase II genes of this fungus and S. schenckii-related species, such as S. schenckii var. luriei and Ceratocystis stenoceras.
OBJECTIVE: To develop a PCR-based identification system that can distinguish S. schenckii from its related species for clinical diagnosis of sporotrichosis.
METHODS: Based on the nucleotide sequences of the DNA topoisomerase II genes of S. schenckii, S. schenckii var. luriei and C. stenoceras, three gene-specific primers (SSHF31 and SSHR97 for S. schenckii, and SSLF64 for S. schenckii var. luriei) were designed and evaluated for their specificities in PCR amplifications.
RESULTS: The primer set SSHF31/SSHR97 amplified PCR products of 663-817 bp from S. schenckii and approximately 660 bp from S. schenckii var. luriei, while SSLF64/SSHR97 exclusively amplified a major product of 305 bp from S. schenckii var. luriei.
CONCLUSION: PCR targeting the DNA topoisomerase II gene specifically and rapidly distinguished S. schenckii from its related species.
OBJECTIVE: To develop a PCR-based identification system that can distinguish S. schenckii from its related species for clinical diagnosis of sporotrichosis.
METHODS: Based on the nucleotide sequences of the DNA topoisomerase II genes of S. schenckii, S. schenckii var. luriei and C. stenoceras, three gene-specific primers (SSHF31 and SSHR97 for S. schenckii, and SSLF64 for S. schenckii var. luriei) were designed and evaluated for their specificities in PCR amplifications.
RESULTS: The primer set SSHF31/SSHR97 amplified PCR products of 663-817 bp from S. schenckii and approximately 660 bp from S. schenckii var. luriei, while SSLF64/SSHR97 exclusively amplified a major product of 305 bp from S. schenckii var. luriei.
CONCLUSION: PCR targeting the DNA topoisomerase II gene specifically and rapidly distinguished S. schenckii from its related species.