Proteomics. Clinical applications · 2025
Proteomic Insights Into the Pathogenesis of Intracerebral Hemorrhage: The Role of Blend Sign in Hematoma Expansion.
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Hang Hang, Liu Shanpeng, Wang Likun, Zhang Linshan, Liu Cuiying, Xu Baohui, Zhao Heng, Wu Guofeng
Аннотация
OBJECTIVE: The "blend sign" is a critical CT imaging marker for predicting hematoma expansion in intracerebral hemorrhage (ICH). This study aimed to elucidate its underlying pathological mechanisms by comparing proteomic profiles between hyperdense and hypodense regions within the hematoma.
METHODS: Hematoma samples from nine ICH patients exhibiting the blend sign were obtained via minimally invasive puncture. Isotope-labeled proteomics and bioinformatic analyses were performed to identify differentially expressed proteins (DEPs), which were further validated by Western blotting and ELISA.
RESULTS: A total of 77 DEPs were identified, including 66 upregulated and 11 downregulated in hyperdense regions compared to hypodense areas. Functional enrichment analysis revealed significant involvement of inflammatory responses, apoptosis, oxidative stress, and metabolic dysregulation. Notably, cytochrome C, growth-associated protein 43 (GAP43), and tau were markedly upregulated in hyperdense regions.
CONCLUSIONS: The blend sign is associated with region-specific molecular changes involving inflammation, apoptosis, and metabolic alterations. These findings provide mechanistic insights into hematoma heterogeneity and lay a foundation for future studies exploring their role in hematoma expansion and clinical outcomes.
METHODS: Hematoma samples from nine ICH patients exhibiting the blend sign were obtained via minimally invasive puncture. Isotope-labeled proteomics and bioinformatic analyses were performed to identify differentially expressed proteins (DEPs), which were further validated by Western blotting and ELISA.
RESULTS: A total of 77 DEPs were identified, including 66 upregulated and 11 downregulated in hyperdense regions compared to hypodense areas. Functional enrichment analysis revealed significant involvement of inflammatory responses, apoptosis, oxidative stress, and metabolic dysregulation. Notably, cytochrome C, growth-associated protein 43 (GAP43), and tau were markedly upregulated in hyperdense regions.
CONCLUSIONS: The blend sign is associated with region-specific molecular changes involving inflammation, apoptosis, and metabolic alterations. These findings provide mechanistic insights into hematoma heterogeneity and lay a foundation for future studies exploring their role in hematoma expansion and clinical outcomes.
Предметные рубрики MeSH
Cerebral HemorrhageProteomicsHematomaTomography, X-Ray ComputedComputational BiologyBiomarkersGene Expression RegulationStereotaxic TechniquesBiopsy, NeedleDisease ProgressionHumansMaleFemaleMiddle AgedAgedAged, 80 and overHemorrhagic StrokeSeverity of Illness IndexProtein Interaction MapsProtein Interaction MappingCytochromes cGAP-43 Proteintau ProteinsApoptosisOxidative Stress