[Characteristics of the virulence of a strain of S. typhimurium and its glycerin mutant in a HEp-2 cell line model].

Sensitivity of the HEp-2 cell culture to the infection with the virulent salmonella strains was shown; active penetration and multiplication of bacteria in the monolayer cells indicated this. Gly 90, a glycerine mutant defective by glycerokinase, obtained under the action of ethylmethanesulphonate, displayed a distinct difference from the initial virulent strains of salmonella by decreased invasive properties and the absence of any capacity to multiplication in the epithelial cells. The data on the avirulence of the glycerine mutant obtained on a model in vitro confirmed the observations carried out in vivo in intraperitoneal infection of albino mice (LD50 = 1-10(7) cells) and in keratoconjunctival infection of guinea pigs.