International immunopharmacology · 2025
Significance of urinary and serum neutrophil gelatinase-associated Lipocalin in Antineutrophil cytoplasmic antibody-associated Vasculitis with glomerulonephritis.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Hao-Miao, Xie Li-Yuan, Chen Tong, Qiu Xian-Ying, Li Yu-Nan, Chen Hong-Shan, Gu Qiu-Hua, Bi Jun, Yan Tie-Kun, Jia Jun-Ya, Xu Peng-Cheng
Аннотация
BACKGROUND: Neutrophil gelatinase-associated lipocalin (NGAL) is primarily secreted by neutrophils and renal tubular epithelial cells. Both neutrophils activation and kidney injury are critical characteristics of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), however, the significance of serum and urinary NGAL in AAV remains to be elucidated.
METHODS: Clinical and laboratory data were collected from 134 patients diagnosed with AAV. The expression of NGAL and the neutrophil marker CD15 was detected using immunohistochemistry (IHC) and immunofluorescence (IF) in kidney biopsy samples.
RESULTS: Both serum and urinary NGAL in AAV were higher than normal controls. Patients with elevated serum NGAL had higher Birmingham vasculitis activity score (BVAS), white blood cells and neutrophils compared to patients with normal serum NGAL. Urinary NGAL correlated with estimated glomerular filtration rate (eGFR) in multivariable correlation analysis. Urinary NGAL was associated with both crescent formation in glomeruli and infiltration of inflammatory cells within the interstitium. IHC and IF demonstrated NGAL was primarily expressed in renal tubular, with additional localization observed around the glomerulus. NGAL partially co-localized with CD15-positive multinuclear cells, implying neutrophils was another source of NGAL. Receiver operating characteristic (ROC) analysis showed urinary NGAL is a predictive marker for crescentic glomerulonephritis. Furthermore, elevated urinary NGAL were correlated with poor renal prognosis.
CONCLUSION: In active AAV, serum NGAL serves as an indicator of disease activity, while urinary NGAL functions as a biomarker for the severity and prognosis of kidney injury. Urinary NGAL not only reflects interstitial tubular injury but also indicates the extent of crescent formation of glomeruli.
METHODS: Clinical and laboratory data were collected from 134 patients diagnosed with AAV. The expression of NGAL and the neutrophil marker CD15 was detected using immunohistochemistry (IHC) and immunofluorescence (IF) in kidney biopsy samples.
RESULTS: Both serum and urinary NGAL in AAV were higher than normal controls. Patients with elevated serum NGAL had higher Birmingham vasculitis activity score (BVAS), white blood cells and neutrophils compared to patients with normal serum NGAL. Urinary NGAL correlated with estimated glomerular filtration rate (eGFR) in multivariable correlation analysis. Urinary NGAL was associated with both crescent formation in glomeruli and infiltration of inflammatory cells within the interstitium. IHC and IF demonstrated NGAL was primarily expressed in renal tubular, with additional localization observed around the glomerulus. NGAL partially co-localized with CD15-positive multinuclear cells, implying neutrophils was another source of NGAL. Receiver operating characteristic (ROC) analysis showed urinary NGAL is a predictive marker for crescentic glomerulonephritis. Furthermore, elevated urinary NGAL were correlated with poor renal prognosis.
CONCLUSION: In active AAV, serum NGAL serves as an indicator of disease activity, while urinary NGAL functions as a biomarker for the severity and prognosis of kidney injury. Urinary NGAL not only reflects interstitial tubular injury but also indicates the extent of crescent formation of glomeruli.