Investigative ophthalmology & visual science · 2009
Impaired angiogenic response in the corneas of mice lacking osteopontin.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fujita Norihito, Fujita Shuko, Okada Yuka, Fujita Kyoko, Kitano Ai, Yamanaka Osamu, Miyamoto Takeshi, Kon Shigeyuki, Uede Toshimitsu, Rittling Susan R, Denhardt David T, Saika Shizuya
Аннотация
PURPOSE: To investigate the effects of loss of osteopontin (OPN) in the development of neovascularization in corneal stroma in mice. Cell culture study was also conducted to clarify the effects of OPN in transforming growth factor (TGF) beta1-driven cell signaling and expression of vascular endothelial growth factor (VEGF).
METHODS: Ocular fibroblasts from wild-type and OPN-null mice were used to study the role of OPN in TGFbeta1 signal and VEGF expression. The effect of the absence of OPN on corneal neovascularization was evaluated in mice.
RESULTS: In ocular fibroblast culture, loss of OPN attenuated TGFbeta1 signals (Smad3 and p38) and reduced expression of VEGF. Loss of OPN attenuated neovascularization in corneal stroma in mice.
CONCLUSIONS: OPN is involved in VEGF expression in cultured fibroblasts and is required for neovascularization in corneal stroma in vivo.
METHODS: Ocular fibroblasts from wild-type and OPN-null mice were used to study the role of OPN in TGFbeta1 signal and VEGF expression. The effect of the absence of OPN on corneal neovascularization was evaluated in mice.
RESULTS: In ocular fibroblast culture, loss of OPN attenuated TGFbeta1 signals (Smad3 and p38) and reduced expression of VEGF. Loss of OPN attenuated neovascularization in corneal stroma in mice.
CONCLUSIONS: OPN is involved in VEGF expression in cultured fibroblasts and is required for neovascularization in corneal stroma in vivo.
Предметные рубрики MeSH
AnimalsAnimals, NewbornCells, CulturedCorneaCorneal NeovascularizationCorneal StromaFibroblastsMacrophages, PeritonealMiceMice, Inbred C57BLMice, KnockoutOsteopontinPlatelet Endothelial Cell Adhesion Molecule-1RNA, MessengerReverse Transcriptase Polymerase Chain ReactionSignal TransductionTransforming Growth Factor beta1Up-RegulationVascular Endothelial Growth Factor A