Virchows Archiv : an international journal of pathology · 2023
Digital spatial profiling of CD4+ T cells in classic Hodgkin lymphoma.
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Takeuchi Mai, Miyoshi Hiroaki, Semba Yuichiro, Yamada Kyohei, Nakashima Kazutaka, Sato Kensaku, Furuta Takuya, Moritsubo Mayuko, Ogura Yusuke, Tanaka Ken, Imamoto Teppei, Arakawa Fumiko, Kohno Kei, Ohshima Koichi
Аннотация
Classic Hodgkin lymphoma (CHL) harbors a small number of Hodgkin-Reed-Sternberg (HRS) cells scattered among numerous lymphocytes. HRS cells are surrounded by distinct CD4+ T cells in a rosette-like manner. These CD4+ T cell rosettes play an important role in the tumor microenvironment (TME) of CHL. To elucidate the interaction between HRS cells and CD4+ T cell rosettes, we completed digital spatial profiling to compare the gene expression profiles of CD4+ T cell rosettes and other CD4+ T cells separated from the HRS cells. Immune checkpoint molecules including OX40, programed cell death-1 (PD-1), and cytotoxic T lymphocyte associated protein 4 (CTLA-4) expression was higher in CD4+ T cell rosettes compared to other CD4+ T cells. Immunohistochemistry confirmed variable PD-1, CTLA-4, and OX40 expression in the CD4+ T cell rosettes. This study introduced a new pathological approach to study the CHL TME, and provided deeper insight into CD4+ T cells in CHL.