Genetic variations of STAT3 gene and their role in hodgkin lymphoma incidence.

BACKGROUND: Previous research has associated STAT3 with cancer development and progression, highlighting its role in supporting precise immune surveillances and regulating pathogenic inflammations. STAT3 is emerging as a crucial transcription factor in the battle of the host against tumors. However, the specific effects of this gene on Hodgkin lymphoma (HL) remain unexplored. OBJECTIVES: This study emphasizes the role of STAT3 gene expression and polymorphisms, particularly SNPs rs744166 A/G and rs72823022 T/C, with an increased incidence in Iraqi patients diagnosed with this type of lymphoma. MATERIALS AND METHODS: A study at the Baghdad Cancer/Health Directorate/Baghdad/ Iraq involved 100 participants (50 HL and 50 controls) Hematological parameters, along with serum levels of urea and creatinine, were measured in both groups. Quantitative reverse transcription PCR (RT-qPCR) was used to assess STAT3 gene expression. Molecular analyses were performed using polymerase chain reaction (PCR). RESULTS: The results showed that The mean fold change in STAT3 gene expression (2-ΔΔCt) was higher in HL patients (4.716) than in the control group. According to the studied SNPs, some genotypes and mutant alleles appeared to have significant protective effects, whereas others appeared to be risk factors that play a significant role in increasing HL incidence. CONCLUSIONS: The results of our investigation revealed that genetic variations in STAT3 may be associated with HL incidence, highlighting it as a possible target for diagnosis and therapeutic intervention.