Revista da Sociedade Brasileira de Medicina Tropical · 2023
Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study.
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Aquino Samuel Ricarte de, Diniz Lucyo Flávio Bezerra, Nunes Sávio Luiz Pereira, Silva Roberta Lane de Oliveira, Gouveia Gisele Veneroni, Gouveia João José Simoni, Sales Kamila Gaudêncio da Silva, Dantas-Torres Filipe, Carmo Rodrigo Feliciano do
Аннотация
BACKGROUND: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.
METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.
RESULTS: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six.
CONCLUSIONS: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.
METHODS: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.
RESULTS: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six.
CONCLUSIONS: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.