Transcranial 810 nm Pulsed Photobiomodulation Improves Learning and Reduces Aβ42 Burden in APP/PS1 Mouse Model of Alzheimer's Disease.

BACKGROUND

Alzheimer's disease (AD) is a leading cause of dementia in older adults, and effective and widely applicable treatment options remain limited. Photobiomodulation (PBM) has shown promise for AD. However, reported estimates of the delivered dose after transcranial propagation vary widely, limiting translation from animal models to clinical settings.

OBJECTIVE

Building on our team's prior clinical findings, this study evaluated whether an 810 nm/10 Hz pulsed PBM regimen improves cognitive performance and reduces Aβ42 burden in APP/PS1 mice.

METHODS

APP/PS1 mice received PBM using an 810 nm LED pulsed at 10 Hz. Irradiation was delivered for 540 sec/day, 6 days/week, for 7 weeks, with a scalp-surface power density of 0.025 W/cm2 and an energy density of 13.5 J/cm2. Cognitive function was evaluated using the Morris water maze, and Aβ42 burden was quantified by immunofluorescence.

RESULTS

Cortical and hippocampal Aβ42 plaque burden was reduced, p < 0.01. Exploratory correlation analyses suggested an association between hippocampal Aβ42 plaque number and reversal-learning performance in the histological subset, p = 0.02. The microglia-Aβ42 colocalization ratio increased by 7.53%, p = 0.03, indicating enhanced spatial association between microglia and Aβ42 after PBM.

CONCLUSIONS

These findings support further evaluation of this 810 nm/10 Hz pulsed PBM regimen in AD mouse models and highlight the value of standardized PBM parameter reporting in preclinical studies.