Saliva based non invasive screening of Oral Submucous Fibrosis using ATR-FTIR spectroscopy.

Oral Submucous Fibrosis (OSMF) is a type of precancerous condition of Oral cancer and considered to have the greatest malignant potential. Biopsy is an ultimate option for the conformation of the malignancy. But the invasiveness of the procedure makes it interdict. Therefore, there is an urgent need to identify effective screening and diagnostic methods which would be less invasive, rapid, more accurate and cost effective. Here, we used Attenuated Total Reflection- Fourier transform infrared spectroscopy (ATR-FTIR) with Chemometric analysis coupled with estimation of total salivary protein to discriminate OSMF and Healthy Control (HC). The present study showed the specific Infrared spectrum for OSMF patients, which was specifically differentiated from HC based on the spectral shift of proteins/amide II, carbohydrate and nucleic acid using Principal Component Analysis (PCA) and Hierarchical Clustering Analysis (HCA) with small data sets. ATR-FTIR spectroscopy of saliva coupled with total protein estimation can be used to discriminate between OSMF and HC. However, large sample size should be needed to evaluate the ATR-FTIR for consideration as a screening tool for an early diagnosis OSMF.