FEBS letters · 2001
A role for N-WASP in invasin-promoted internalisation.
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McGee K, Zettl M, Way M, Fällman M
Аннотация
Phagocytosis of Yersinia pseudotuberculosis occurs through interaction of the bacterial protein invasin with beta1-integrins. Here we report that N-WASP plays a role in internalisation of an invasin-expressing, avirulent strain of Y. pseudotuberculosis. Ectopic expression of N-WASP mutants, which affect recruitment of the Arp2/3 complex to the phagosome, reduces uptake of Yersinia. In addition, expression of the Cdc42/Rac-binding (CRIB) region of N-WASP has an inhibitory effect on uptake. Using GFP-tagged Rho GTPase mutants, we provide evidence that Rac1, but not Cdc42, is important for internalisation. Furthermore, activated Rac1 rescues Toxin B, CRIB and Src family kinase inhibitor PP2-mediated impairment of uptake. Our observations indicate that invasin-mediated phagocytosis occurs via a Src and WASP family-dependent mechanism(s), involving the Arp2/3 complex and Rac, but does not require Cdc42.
Предметные рубрики MeSH
Adhesins, BacterialBacterial ProteinsCarrier ProteinsCytoskeletal ProteinsDNA, ComplementaryDose-Response Relationship, DrugGreen Fluorescent ProteinsHeLa CellsHumansIntracellular Signaling Peptides and ProteinsLuminescent ProteinsMicroscopy, FluorescenceMutationNerve Tissue ProteinsPhagocytosisPlasmidsProtein BindingSignal TransductionTransfectionWiskott-Aldrich Syndrome Protein, NeuronalYersinia pseudotuberculosiscdc42 GTP-Binding Proteinrac GTP-Binding Proteinssrc-Family Kinases