[Dysregulation of cooperative interactions of immunocytes and eosinophils in the mechanism of development of eosinophilia in Opisthorhis felineus invasion].

The authors studied the levels of mononuclear cell production of eosinophil-specific cytokines (IL-3, IL-5), the serum levels of eotaxin by enzyme immunoassay; the expression of the eosinophilic cell receptor apparatus by flow cytofluorometry in patients with acute and chronic Opisthorchis invasion. Eosinophilia-associated Opisthorchis invasion was found to be accompanied by a pronounced change in the serum production of the key cytokine regulators of eosinophilic homeostasis (elevated IL-3 and IL-5 levels) and eotaxin by peripheral blood mononuclear leukocytes. There was an increase in the number of receptor structures to eosinophil-specific cytokines (IL-5R-, IL-3R-, and CCR3-positive cells) in patients with opisthorchiasis. In vitro incubation of the eosinophils, obtained from patients with opisthorchiasis, with the recombinant forms of cytokines (IL-5, IL-3, and eotaxin) demonstrated the decreased expression of IL-5 and IL-3 receptors with the normal presentation of CCR3. With the developed acute helminthiasis, the revealed changes were more pronounced than those observed in chronic Opisthorchis invasion.